Search | Engineering

Subscribe Submit

Home Journals Focus Achievement Fronts About Us 中文版

Resource Type

Journal Article 2

Year

2020 1

2015 1

Keywords

Catharanthus roseus 1

Essential oil 1

Linalool 1

Monoterpenoid 1

NMR 1

Nicotinic acetylcholine receptor 1

metabolomics 1

monoterpenoid indole alkaloids 1

γ-aminobutyric acid type A receptor 1

open ︾

Search scope:

排序： Display mode:

Progress in NMR-based metabolomics of

Qifang PAN,Jingya ZHAO,Yuliang WANG,Kexuan TANG

Frontiers of Agricultural Science and Engineering 2015, Volume 2, Issue 3, Pages 195-204 doi: 10.15302/J-FASE-2015063

Abstract: As the only natural source for a diversity of monoterpenoid indole alkaloids (MIAs), especially the low-abundance

Keywords： Catharanthus roseus monoterpenoid indole alkaloids NMR metabolomics

HTML PDF Collect

Putative Mode of Action of the Monoterpenoids Linalool, Methyl Eugenol, Estragole, and Citronellal on Ligand-Gated Ion Channels Article

Amy S. Li, Akimasa Iijima, Junhao Huang, Qing X. Li, Yongli Chen

Engineering 2020, Volume 6, Issue 5, Pages 541-545 doi: 10.1016/j.eng.2019.07.027

Abstract:

Essential oil has been used as sedatives, anticonvulsants, and local anesthetics in traditional medical remedies; as preservatives for food, fruit, vegetable, and grain storage; and as bio-pesticides for food production. Linalool (LL), along with a few other major components such as methyl eugenol (ME), estragole (EG), and citronellal, are the active chemicals in many essential oils such as basil oil. Basil oil and the aforementioned monoterpenoids are potent against insect pests. However, the molecular mechanism of action of these chemical constituents is not well understood. It is well-known that the γ-aminobutyric acid type A receptors (GABA_ARs) and nicotinic acetylcholine receptor (nAChR) are primary molecular targets of the synthetic insecticides used in the market today. Furthermore, GABA_AR-targeted therapeutics have been used in clinics for many decades, including barbiturates and benzodiazepines, to name just a few. In this research, we studied the electrophysiological effects of LL, ME, EG, and citronellal on GABA_AR and nAChR to further understand their versatility as therapeutic agents in traditional remedies and as insecticides. Our results revealed that LL inhibits both GABA_AR and nAChR, which may explain its insecticidal activity. LL is a concentration-dependent, non-competitive inhibitor on GABA_AR, as the half-maximal effective concentration values of γ-aminobutyric acid (GABA) for the rat α1β3γ2L GABA_AR were not affected by LL: (36.2 ± 7.9) µmol·L⁻¹ and (36.1 ± 23.8) µmol·L⁻¹ in the absence and presence of 5 mmol·L⁻¹ LL, respectively. The half-maximal inhibitory concentration (IC₅₀) of LL on GABA_AR was approximately 3.2 mmol·L⁻¹. Considering that multiple monoterpenoids are found within the same essential oil, it is likely that LL has a synergistic effect with ME, which has been previously characterized as both a GABA_AR agonist and a positive allosteric modulator, and with other monoterpenoids, which offers a possible explanation for the sedative and anticonvulsant effects and the insecticidal activities of LL.